In order to investigate the histopathological, mechanism of Rompun-induced shock, the development of mammary carcinoma, the numerical changes and the morphological findings of the mast cells appeared in the carcinoma were microscopically observed in the rat treated with DMBA and each chemical of compound 48/80 and Rompun. Also mast cell degranulation induced by Rompun was observed with electron microscope.
The results observed were summarized as follows:
Tumor appeared in 100% of the animals. Tumors grevv more rapidly to 10×10㎜ in rats depleted of mast cells (37.7±4.2 days) than was observed in the control group (42.5±4.7 days) (p<0.005). The mean number of tumors per rat was 2.8±1.3 in the compoand 48/80treated group in contrast to 3.4±1.3 in the control group. No significant difference was apparent in tlie tumor induction time of Rompun treated group compared with the compound 48/80-treated group, but the tumor measuring at least 10×10㎜ appcared more quickly in the Rompun-treated group than in the control group (p<0.005).
The numbers of mast cells in the control group were inclined to increase significnntly according to the mammary tumor development (p<0.005). In contrast, the mast cells were fewer significantly in the compound 48/80-treated group and Rompun-treated group than in the control group (p<0.005). The numbers of mast cells in the compound 48/80-treated group and Rompun-treated group were inclined to reduce significantly according to the stages of the mammary carcinoma growth in contrast to the control group respectively.
The ultrastructural morphologies of mast cells at 30 minutes after Rompun injection were appeared many normal granules in the cytoplasm, but many normal and degranulated granules were scattered along the cell membrane. And at 1 hour after Rompun injection mast cell granules were disappeared nearly or rarely seen, many long cytoplasmic projections were folded back to adhere to their own surface membrane, and mast cells resulted in a reduced size of these cells.
Otherwise, compound 48/80 caused extensive degranulation of mast cells by disrupting cell membrane, but mast cell degranulation by Rompun was observed exocytosis of granules through a channel.
From the above results, it is concluded that the Rompun may give rise to the dealth of animals as a shock caused by mast cell degranulation.
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